Experimental left pneumonectomy in pigs. Procedure and management

Background Because there is no detailed description of procedures and perioperative management of major pulmonary resections in swine, we reviewed our experience to delineate the most effective practice in performing left pneumonectomy. Materials and methods Analysis of 11 consecutive left pneumonectomies. Animal data, operative reports, anesthesia records, and perioperative facts were evaluated. Follow-up information until postoperative day 60, methods of care-taking, therapy administration, and all the stabling aspects were systematically assessed. The investigation was aimed at highlighting those procedural steps or details which make the difference in optimizing the available resources (animals, instruments, and personnel). No statistical analysis was performed considering data characteristics and the descriptive nature of information. Results Surgery requires a median time of 2 h and 16 min; two operators and one anesthesiologist represent the basic team. Circulators' number depends on goals to accomplish. The most straightforward procedure requires careful dissection of the pulmonary ligament (limited view), pulmonary veins (low variability), pulmonary artery (delicate), and finally bronchus (no variability observed). The key factors for good anesthesia management have been identified: sedation by caregivers, preoxygenation before induction of general anesthesia, high respiratory rates with low tidal volume after pneumonectomy, and noninvasive ventilation after extubation. Antibiotic prophylaxis has been performed. Postoperative care must be continuous until animals are able to stand up, afterward "preventive noncurative," and always animal friendly. Ideas for minimally stressful therapy administration are helpful. Conclusions After the delineation of this methodology, the compliance to a routine practice allowed us to reduce time, stress, and cost; quality and quantity of possible research increased

Wavelength locking of silicon photonics multiplexer for DML-based WDM transmitter

We present a wavelength locking platform enabling the feedback control of silicon (Si) microring resonators (MRRs) for the realization of a 4 × 10 Gb/s wavelength-division-multiplexing (WDM) transmitter. Four thermally tunable Si MRRs are employed to multiplex the signals generated by four directly modulated lasers (DMLs) operating in the L-band, as well as to improve the quality of the DMLs signals. Feedback control is achieved through a field-programmable gate array controller by monitoring the working point of each MRR through a transparent detector integrated inside the resonator. The feedback system provides an MRR wavelength stability of about 4 pm (0.5 GHz) with a time response of 60 ms. Bit error rate (BER) measurements confirm the effectiveness and the robustness of the locking system to counteract sensitivity degradations due to thermal drifts, even under uncooled operation conditions for the Si chip

PIXAPP Photonics Packaging Pilot Line development of a silicon photonic optical transceiver with pluggable fiber connectivity

This paper demonstrates how the PIXAPP Photonics Packaging Pilot Line uses its extensive packaging capabilities across its European partner network to design and assemble a highly integrated silicon photonic-based optical transceiver. The processes used are based on PIXAPP's open access packaging design rules or Assembly Design Kit (ADK). The transceiver was designed to have the Tx and Rx elements integrated on to a single silicon photonic chip, together with flipchip control electronics, hybrid laser and micro-optics. The transceiver used the on-chip micro-optics to enable a pluggable fiber connection, avoiding the need to bond optical fibers directly to the photonic chip. Finally, the packaged transceiver module was tested, showing 56 Gb/s loop-back modulation and de-modulation, validating both the transmitter and receiver performance

Measurement-based models of a 40 Gb/s modulator and its electrical driver for joint transmitter design

A measurements-based model of Mach-Zehnder electrooptic modulator and of its electrical driverare proposed.These models are essential for concurrent design of the two devices,and allow careful optimisation ofthe overall performance of the 40 Gb/s transmitter.The design of a travelling wave driver amplifier for a given fabricated modulator has been performed by using the proposed models.An improvement of about 6 dB has beenobtained for the extinction ratio with respect to a previously designed 7 Vpp driver

A Mach-Zehnder modulator model for the design of optical-fiber analog transmitters

A measurement-based model of Mach-Zehnder electrooptic modulators is proposed that allows the design of a transmitter for optical analog links. The model, extracted for a modulator fabricated at Avanex laboratories, has been used to design a circuit in monolithic CMOS technology that provides broad-band improvement of spurious-free dynamic range. © 2011 IEEE

Observational analysis on inflammatory reaction to talc pleurodesis: Small and large animal model series review

Talc pleurodesis has been associated with pleuropulmonary damage, particularly long-term damage due to its inert nature. The present model series review aimed to assess the safety of this procedure by examining inflammatory stimulus, biocompatibility and tissue reaction following talc pleurodesis. Talc slurry was performed in rabbits: 200 mg/kg checked at postoperative day 14 (five models), 200 mg/kg checked at postoperative day 28 (five models), 40 mg/kg, checked at postoperative day 14 (five models), 40 mg/kg checked at postoperative day 28 (five models). Talc poudrage was performed in pigs: 55 mg/kg checked at postoperative day 60 (18 models). Tissue inspection and data collection followed the surgical pathology approach currently used in clinical practice. As this was an observational study, no statistical analysis was performed. Regarding the rabbit model (Oryctolagus cunicoli), the extent of adhesions ranged between 0 and 30%, and between 0 and 10% following 14 and 28 days, respectively. No intraparenchymal granuloma was observed whereas, pleural granulomas were extensively encountered following both talc dosages, with more evidence of visceral pleura granulomas following 200 mg/kg compared with 40 mg/kg. Severe florid inflammation was observed in 2/10 cases following 40 mg/kg. Parathymic, pericardium granulomas and mediastinal lymphadenopathy were evidenced at 28 days. At 60 days, from rare adhesions to extended pleurodesis were observed in the pig model (Sus Scrofa domesticus). Pleural granulomas were ubiquitous on visceral and parietal pleurae. Severe spotted inflammation among the adhesions were recorded in 15/18 pigs. Intraparenchymal granulomas were observed in 9/18 lungs. Talc produced unpredictable pleurodesis in both animal models with enduring pleural inflammation whether it was performed via slurry or poudrage. Furthermore, talc appeared to have triggered extended pleural damage, intraparenchymal nodules (porcine poudrage) and mediastinal migration (rabbit slurry)

Preliminary results on skin incision, tissue damage and wound healing on in-vivo porcine skin model: a comparison between different incision methods, based on surgical, histological and immunohistochemical results. Part One: surgical evaluations

Aim of this study was to compare quality of incisions and lateral thermal injury (LTI) produced by Diode Laser (DL), CO2 Laser (CO2L), Thulium Laser (TL), monopolar electrosurgery (MES) and Steel Scalpel (SS) and their effects on the reepithelialisation of incisional wounds of porcine skin